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g l brain heart infusion  (MedChemExpress)


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    Structured Review

    MedChemExpress g l brain heart infusion
    G L Brain Heart Infusion, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g+l+brain+heart+infusion/Brain+Heart+Infusion+Broth/pmc12772305-151-8-19
    Average 94 stars, based on 7 article reviews
    g l brain heart infusion - by Bioz Stars, 2026-10
    94/100 stars

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    Modification:

    Article Title: Prevotella copri facilitates wound healing in mice through the sphingosine-CerS1-ceramide metabolic pathway
    Article Snippet: .. A modified BHI broth was prepared containing 38.5 g/L brain heart infusion (Huankai Microbial, China), 1 g/L L-cysteine hydrochloride (MCE), 10 mg/L hemin chloride (MCE), and 1 mg/L vitamin K1 (MCE). ..

    Article Title: Prevotella copri facilitates wound healing in mice through the sphingosine-CerS1-ceramide metabolic pathway.
    Article Snippet: .. A modified BHI broth was prepared containing 38.5 g/L brain heart infusion (Huankai Microbial, China), 1 g/L L-cysteine hydrochloride (MCE), 10 mg/L hemin chloride (MCE), and 1 mg/L vitamin K1 (MCE). ..



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    Mutational analysis of CmrR. ( A ) C. difficile ∆ cmrR strains carrying the indicated plasmids were assayed for surface motility on <t>BHIS-1.5%</t> agar with the indicated concentration of ATc. Motility was quantified as the diameter after 7 days of growth. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; P < 0.01, **; two-way ANOVA compared to pCmrR with the respective ATc concentration using Dunnett’s post-test. ( B ) A bacterial two-hybrid assay in C. difficile was used to assess interactions between mutant CmrR proteins. Luciferase signal was measured from strains carrying plasmids with the indicated allele of cmrR fused to each half of split luciferase. The positive control strain expresses full-length luciferase enzyme, and the negative control strain expresses split luciferase fragments without a translational fusion. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; Brown-Forsythe and Welch ANOVA compared to CmrR with Dunnett’s T3 post-test.
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    Mutational analysis of CmrR. ( A ) C. difficile ∆ cmrR strains carrying the indicated plasmids were assayed for surface motility on <t>BHIS-1.5%</t> agar with the indicated concentration of ATc. Motility was quantified as the diameter after 7 days of growth. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; P < 0.01, **; two-way ANOVA compared to pCmrR with the respective ATc concentration using Dunnett’s post-test. ( B ) A bacterial two-hybrid assay in C. difficile was used to assess interactions between mutant CmrR proteins. Luciferase signal was measured from strains carrying plasmids with the indicated allele of cmrR fused to each half of split luciferase. The positive control strain expresses full-length luciferase enzyme, and the negative control strain expresses split luciferase fragments without a translational fusion. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; Brown-Forsythe and Welch ANOVA compared to CmrR with Dunnett’s T3 post-test.
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    Mutational analysis of CmrR. ( A ) C. difficile ∆ cmrR strains carrying the indicated plasmids were assayed for surface motility on BHIS-1.5% agar with the indicated concentration of ATc. Motility was quantified as the diameter after 7 days of growth. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; P < 0.01, **; two-way ANOVA compared to pCmrR with the respective ATc concentration using Dunnett’s post-test. ( B ) A bacterial two-hybrid assay in C. difficile was used to assess interactions between mutant CmrR proteins. Luciferase signal was measured from strains carrying plasmids with the indicated allele of cmrR fused to each half of split luciferase. The positive control strain expresses full-length luciferase enzyme, and the negative control strain expresses split luciferase fragments without a translational fusion. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; Brown-Forsythe and Welch ANOVA compared to CmrR with Dunnett’s T3 post-test.

    Journal: Journal of Bacteriology

    Article Title: Characterization of two co-regulated response regulators in Clostridioides difficile

    doi: 10.1128/jb.00177-25

    Figure Lengend Snippet: Mutational analysis of CmrR. ( A ) C. difficile ∆ cmrR strains carrying the indicated plasmids were assayed for surface motility on BHIS-1.5% agar with the indicated concentration of ATc. Motility was quantified as the diameter after 7 days of growth. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; P < 0.01, **; two-way ANOVA compared to pCmrR with the respective ATc concentration using Dunnett’s post-test. ( B ) A bacterial two-hybrid assay in C. difficile was used to assess interactions between mutant CmrR proteins. Luciferase signal was measured from strains carrying plasmids with the indicated allele of cmrR fused to each half of split luciferase. The positive control strain expresses full-length luciferase enzyme, and the negative control strain expresses split luciferase fragments without a translational fusion. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; Brown-Forsythe and Welch ANOVA compared to CmrR with Dunnett’s T3 post-test.

    Article Snippet: C. difficile was grown in pre-reduced supplemented (BHIS) medium (37 g/L Bacto brain heart infusion, 5 g/L yeast extract) with 0.1% L-cysteine or Tryptone Yeast (TY) medium (30 g/L Bacto tryptone, 20 g/L yeast extract, 1 g/L thioglycolate) with antibiotics when appropriate.

    Techniques: Concentration Assay, Two Hybrid Assay, Mutagenesis, Luciferase, Positive Control, Negative Control

    Mutational Analysis of CmrT. ( A ) C. difficile ∆ cmrR ∆ cmrT strains carrying overexpression plasmids with the indicated cmrT alleles were assayed for surface motility on BHIS-1.5% agar with the indicated concentration of ATc. Motility was quantified as the diameter after 7 days of growth. Data from three biological replicates are plotted with mean and SD. P < 0.0001, #; two-way ANOVA compared to pCmrT with Dunnett’s posttest. ( B ) The C. difficile two-hybrid assay was used to assess interactions between CmrT variants. Luciferase signal was measured from strains carrying plasmids encoding the indicated allele of cmrT fused to each half of the split luciferase. The positive control strain expresses full-length luciferase enzyme, and the negative control strain expresses split luciferase fragments without translational fusions. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; P < 0.01, **; one-way ANOVA compared to CmrT with Dunnett’s post-test.

    Journal: Journal of Bacteriology

    Article Title: Characterization of two co-regulated response regulators in Clostridioides difficile

    doi: 10.1128/jb.00177-25

    Figure Lengend Snippet: Mutational Analysis of CmrT. ( A ) C. difficile ∆ cmrR ∆ cmrT strains carrying overexpression plasmids with the indicated cmrT alleles were assayed for surface motility on BHIS-1.5% agar with the indicated concentration of ATc. Motility was quantified as the diameter after 7 days of growth. Data from three biological replicates are plotted with mean and SD. P < 0.0001, #; two-way ANOVA compared to pCmrT with Dunnett’s posttest. ( B ) The C. difficile two-hybrid assay was used to assess interactions between CmrT variants. Luciferase signal was measured from strains carrying plasmids encoding the indicated allele of cmrT fused to each half of the split luciferase. The positive control strain expresses full-length luciferase enzyme, and the negative control strain expresses split luciferase fragments without translational fusions. Data from three biological replicates are plotted with mean and SD. P < 0.05, *; P < 0.01, **; one-way ANOVA compared to CmrT with Dunnett’s post-test.

    Article Snippet: C. difficile was grown in pre-reduced supplemented (BHIS) medium (37 g/L Bacto brain heart infusion, 5 g/L yeast extract) with 0.1% L-cysteine or Tryptone Yeast (TY) medium (30 g/L Bacto tryptone, 20 g/L yeast extract, 1 g/L thioglycolate) with antibiotics when appropriate.

    Techniques: Over Expression, Concentration Assay, Two Hybrid Assay, Luciferase, Positive Control, Negative Control